Isolation and identification of lignin degrading bacteria residing in mandeepkhol cave of Chhattisgarh: A RAPD Study

Sur, Arunima ; Nayak, Kush Kumar ; Parkhey, Piyush ; Sahu, Reecha

Abstract

Lignin is biopolymer synthesized by plants and it consists upto 30% dry mass weight of any woody tissue. It is also known as second most abundant source of carbon after cellulose but still has a major problem, i.e. very tough to degrade. Thus, in present work authors were studied about the degradation of lignin by bacteria. The paper reports isolation, screening and identification of lignin degrading bacteria from collected samples. The bacterial strains were isolated from a cave that is located in the Chhattisgarh state of India in the mountain range of Salewara which have a temperature range between 26-28C. A total of 32 bacterial isolates were studied and their RAPD analysis was performed and it was observed that Brevundimonas diminuta, Bacillus thuringiensis and Bacillus cereus were isolated in lignin- rich media which showed evidence of high lignin degradation.

Keyword(s)

16S rDNA; Bioleaching; Lignin; RAPD

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