Impact of sanitizers on quality of Lipase and Triglyceride analytes in clinical laboratory

Sharma, Anjali ; Maksane, Shalini ; Das, Jhuma ; Kumar, Sudhir ; Birdi, Amandeep ; Tomo, Sojit ; Yadav, Dharmveer ; Sharma, Praveen

Abstract

Use of sanitizers in diagnostic centres causes derangement in quality control values of lipase and triglycerides (TG) analytes during COVID-19. Our study provides a practical insight into the type of sanitizers to be used in a laboratory. Performance Verifier (PV) and reagents (lipase and TG) were contaminated with sanitizer and 70% isopropyl alcohol. Groups formed were- PVNet (G1), PV with Sanitizer (G2), PV with 70% Alcohol (G3), Sanitizer contaminated reagent (G4). Controls PV-1 and PV-2 were run. ANOVA and Tukey’s test among groups and between groups were compared. Significant difference in mean PV-1and PV-2 values of TG [PV-1, PV-2 (P <0.0001)] and lipase TG [PV-1 P <0.0001) PV-2 P <0.001] among all tested groups were observed. Between-group analysis showed significantly higher PV-1 and PV-2 values in sanitizer contaminated PV group (P <0.001) compared to Neat PVs (P <0.001), and PVs contaminated with 70% alcohol (P <0.001). sanitizer contaminated PV-1 values were significantly higher when compared to Neat PV-1 (P <0.001)for lipase. It is advised that isopropyl alcohol (70%) should be preferred over glycerol containing sanitizers to reduce pre-analytical errors for lipase and TG estimation.

Keyword(s)

Analytes, Dihydroxyacetone, Hypertriglyceridemia, Quinoneimine

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