Assessment of cranberry bush on MCF-7 human breast cancer cells

KAAN, DİLEK

Abstract

This research aimed to study the therapeutic potential of Viburnum opulus L. (VO) powder extracts on human breast cancer and normal breast epithelial cell lines. The half-maximum inhibitory concentration IC50 value was 51.32 μg/mL for 24 h using an MTT assay. The total apoptotic induction was found to be 40.50% for MCF-7 cells exposed to 100 μg/mL VO powder extract for 48 h. The genotoxic effect in MCF-7 cells was statistically significantly increased in a dose-dependent manner after exposure to VO powder extracts. P1 class glutathione-S-transferase (GSTP1) expression level was noticed protective feature in drug resistance metabolism. GSTP1 activities were found statistically significant differences between all of the groups (**P <0.01). GSTP1 overexpressed in MCF-7 control cells and expression levels decreased in the MCF-7 groups that were treated with VO powder extract at different concentrations of 25, 50 and 100 μg/mL for 24, 48 and 72 h. These results suggest that VO powder extracts suppress the proliferation and GSTP1 expression of MCF-7 cells and this suppression is attributed to both induction of apoptosis and DNA damage. This study provided the information that VO powder extract may be suitable for drug therapy efficacy at the molecular level.

Keyword(s)

Alternative medicine; Apoptosis; Genotoxicity effects; Metabolic protein level

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