Synthesis of iron nanoparticles, its characterization and anti-microbial activity assessment

Sawarn, Ayush ; Singh, Priyanka ; Dangi, Kapil ; Yadav, Pooja ; Kamra Verma, Anita

Abstract

We report the efficacy of the Iron nanoparticles (IONPs) and assessed two different approaches for the synthesis of IONPs i.e. Polyol and co-precipitation method and further, evaluate their antimicrobial properties. Ferrous sulphate heptahydrate salts were reduced with ethylene glycol to obtain IONP and Fe+2 and Fe+3 co-precipitation reaction was performed with KOH at optimum heating. Further, synthesized (IONPs) were characterized by hydrodynamic radii measurement done by DLS clearly indicating the size of IONPs is 79.75nm in polyol based and 135.1 nm in co-precipitation method. The biological efficacy in terms of antimicrobial activity was assessed by the Kirby Bauer method, applied for both Gram-positive and Gram-negative bacteria such as Staphylococcus aureus and Escherichia coli, respectively. The ZOI values i.e. Zone of inhibition diameter was found to be clearly visible in both S. aureus and E. coli, indicating bactericidal activity. Further growth kinetics studies and bacterial genotoxicity was also assessed. Hence, IONPs synthesized are proposed to have great potential as an antibacterial agent and can be used in drug delivery.

Keyword(s)

Bacterial genotoxicity; Co-precipitation method; Growth kinetics; Kirby-Bauer method; Polyol method; Zone of inhibition

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