Geminally biotinylated cyclotriphosphazenes as molecular binding probe

Ma, Xuebin ; Xu, Tingting ; Chen, Wei ; Wang, Rui ; Xu, Zheng ; Chi, Bo ; Ye, Zhiwen

Abstract

Biotinylated cyclotriphosphazenes polymer (g-BTP) with germinal octopus-like molecular shape has been prepared and characterized by 31P-NMR, 1H-NMR and Matrix-assisted laser desorption ionization-time of flight-mass spectrometry (Maldi-Tof-Ms). Analysis of the size distribution of the complexes showed that the aggregated g-BTP/avidin complexes were rather polydispersed and uniform as compared to complexes formed by random coil biotinylated linear polymer (BLP) and avidin. Binding kinetics studies show that both of g-BTP and BLP can bind avidin very quickly. The binding ability as evaluated by Scatchard plot indicates the binding ability of g-BTP to be about 5.5 times that of BLP.


Keyword(s)

Biotinylated cyclotriphosphazenes polymer; binding ability; sensors

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