Interfacial and Thermodynamic Approach of Surfactants with α-Chymotrypsin and Trypsin: A Comparative Study

Banjare, Manoj Kumar

Abstract

The conductivity as well as surface tension measurements have carried out to study the interactions among cetyltrimethyl ammonium bromide (CTAB), sodium dodecyl sulpahe (SDS) with α-chymotrypsin (α-CT) and trypsin in aqueous medium on pH 7.75. The surface parameter i.e., critical micelle concentrations (CMC), the maximum surface excess concentration (Γmax), minimum area per surfactant molecule (Amin) and the surface pressure at CMC (πcmc), and thermodynamic parameters i.e., degree of ionization (α), Gibbs free energy of micellization (ΔG°m), the standard Gibbs energy of adsorption (ΔG°ads), the free energy at air-water interface (ΔGsmim) have been evaluated using surface tension and conductivity measurement. Thermodynamic parameters indicate that enzyme-CTAB/SDS monomeric aggregation started to form micelles at a higher concentration of surfactant to compare with the CMC of pure CTAB/SDS micelles.


Keyword(s)

α-Chymotrypsin, Trypsin, Surfactant, Micellization Behavior, Conductivity, Surface tension.


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