Synthesis of [N-bis(4-methyl benzene-diamino formyl)methyl phosphonic acid] and its europium (III) complex and its application as DNA electrochemical sensor

SONG, Pan ; SHE, Wen Jie ; ZHOU, Tong ; YANG, Tian Lin ; NI, Gang ; NA, Peng Jun

Abstract

The new tripod ligand [N-bis(4-methyl benzene-diamino formyl)methyl phosphonic acid] and its europium (III) complex (Eu(pic)3L) have been synthesized and characterized by TG-DSC, IR spectroscopy, UV-vis spectroscopy, elemental and molar conductance analysis. The experimental results show that the ligand forms the 1:1 complex with europium picrate. The results of  UV-vis spectroscopy and cyclic voltammetry indicate that there are intercalation interaction between DNA and Eu(pic)3L. When the Eu(pic)3L is regarded as DNA hybridization probe and used in the study of DNA electrochemical sensor, no obvious electrochemical signal response could be found with Eu(pic)3L under the effect of modified electrodes single-strand DNA testing. However, obvious electrochemical signal response is found when it is under the effect of double-stranded DNA testing. It indicates that the biosensor can discriminate well between the complementary sequences from the base-mismatched and the non-complementary sequences. So Eu(pic)3L can be used as a biosensor to discriminate between the complementary sequences from the base-mismatched and the non-complementary sequences.

Keyword(s)

Phosphorus-containing tripod ligand; Rare earth complex; DNA electrochemical sensor

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