Nephroprotective Effect of Methanolic Broccoli (Brassica olaracea, var. Italica) Extract on Oxidative-lead Damage in Mice Kidney: Biochemical Parameters and Phatological changes

Raeeszadeh, Mahdieh ; Zobeiri, Ashkan ; Khademi, Nadia ; Mortazavi, Pejman

Abstract

Lead oxidative stress mechanism reduces antioxidant capacity of the cell. Broccoli is a good and important source of antioxidant and mineral compounds. NMRI mice in control group  were given normal drinking water and T1 experimental group received 500 ppm lead acetate in drinking water. The T2, T3 and T4 experimental groups received 300, 400 and 500mg/kg of methanolic broccoli extract, respectively. On the last day, biochemical parameters and oxidative stress were measured in kidney Kidney tissue was used for preparation of sections and pathological studies. The highest concentrations of biochemical parameters  in T1 showed a significant difference in comparison with the control and T4 groups (P<0.01). TAC, CAT and SOD in T1 showed the lowest level, whereas the highest concentration of TAC and SOD were in T4(P=0.032). MDA in T1 was 20.85 ± 0.270 nmol / mgr and the difference with T4 were remarkable(P<0.001).  The highest score of pathological damage to the renal tissue was in T1 and lowes  damage in T4. There was a significant difference between T4 in comparison with the control group(P=0.034). So that’s an interesting achievement the methanolic extract of Broccoli (Brassica olaracea, var. Italica) in dose of 500mg/kg had the best nephroprotective effects against injuries of soluble in lead acetate drinking water.


Keyword(s)

Oxidative Stress, Lead, Kidney, Broccoli


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