Extraction and partial characterization of exopolysaccharides and pigments from cyanobacterium Oscillatoria pseudogeminata G.Schmid

Thangaraj, Ramasamy ; Thajuddin, Nooruddin

Abstract

Cyanobacterial pigments such as chlorophyll, carotenoids and phycobiliproteins are considered as ecofriendly natural colourants used in food and food additives as they have nutraceutical value, non-toxic and non-carcinogenic. The present study focused on extraction, partial characterization of extracellular polysaccharides (EPS), phycocyanin and mycosporin like amino acid (MAA) from a freshwater cyanobacterium Oscillatoria pseudogeminata G.Schmid.The purified isolate of O.pseudogeminatawas deposited and maintained in the NRMC-F, Bharathidasan University. The EPS was extracted withthe help of double volume of ice-cold acetone and confirmed by UV (260 and 280 nm), FTIR and XRD analyses.Phycocyanin and MAA were also extracted from O. pseudogeminata biomass using double distilled water. The UV-VisSpectrophotometric results revealed that 520 and 230 nm peaks represent phycocyanin and MAA, respectively. Further, theprocess of scaling-up the biomass and increase the productivity of EPS, Phycocyanin and MAA from Oscillatoriapseudogeminata is under raceway pond system, already initialed in our laboratory.

Keyword(s)

Biomass; Food colourant; Mycosporin like amino acid; Natural colourants; Phycocyanin

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