Green synthesis of zinc oxide nanoparticle using Pentatropis capensis and its anti-proliferative activity

Abstract

The ZnO nanoparticle synthesized by green synthesis method using aerial parts of Pentatropis capensis was characterized using UV-Vis spectroscopy, FTIR, SEM, and EDAX. The anti proliferative activity was tested against HepG2 and MCF-7 cell lines by MTT assay and the IC₅₀ value was calculated.The absorption peak at 330 nm confirms the presence of ZnO nanoparticle. The FTIR spectra of synthesized ZnO nanoparticle confirm the various groups present in it. The SEM image reveals the size of ZnO nanoparticle, which ranged from 73.4-398.3 nm. The elemental composition of ZnO nanoparticles consists of zinc, carbon, oxygen, potassium, calcium, and chlorine which was determined in EDAX analysis. The IC₅₀ value of anti-proliferative activity was found to be 23.277 µg/mL for HepG2 cell line and 12.926 µg/mL for the MCF-7 cell line. The green synthesized ZnO nanoparticle showed an effective anti-proliferative activity on both HepG2 and MCF cell line.