Effect of combination of fenugreek with insulin and Glimepiride on male reproductive system in Streptozotocin-induced diabetic rats

Abstract

Present study was conducted to assess the effect of fenugreek, insulin and Glimepiride alone and their combination on male reproductive system in diabetic rat. Fifty six male Sprague Dawley rats of uniform age were randomly divided into seven groups. Group 1 and 2 kept as non-diabetic control and Streptozotocin (40 mg/kg i/p single dose)-induced diabetic control; Group 3, 4 and 5 were given treatment with insulin, Glimepiride and fenugreek seed powder, respectively; Group 6 and 7 were given combination of insulin + fenugreek seed powder and Glimepiride + fenugreek seed powder, respectively. At the end of experiment, testes were collected, weighed and used for histopathology and estimation of GSH, TBARS and epididymus was collected for estimation of total sperm count. The concentration of TBARS was increased, while GSH and relative testis weights were decreased in diabetic rats. The histology of testis revealed marked changes in diabetic rats and mild changes in combination treatment groups. The treatment groups showed significant improvement as compared to diabetic rats, while the combination groups showed greatest improvement. The study revealed that addition of fenugreek seed powder to insulin and Glimepiride had positive interaction in improving the male reproductive parameters in streptozotocin-induced diabetic Sprague Dawley rats.