Phytochemical screening, anti-oxidant and anti-inflammatory activities of ethyl acetate extract of Combretum punctatum var. squamosum

Thanzami, K ; Pachuau, V L Thatpuii; Lalremruati, C ; Kakoti, Bibhuti B

Abstract

The objective of this study was to carry out the phytochemical screening of the ethyl acetate extract of Combretum punctatum var. squamosum and to investigate anti-inflammatory and anti-oxidant activities. The phytochemical investigation indicated the presence of flavonoids and steroids in the extract. Anti-inflammatory activity was studied by in vitro inhibition of albumin denaturation and HRBC membrane stabilization methods; in vivo cotton pellet induced granuloma and carrageenan-induced paw oedema studies. The anti-oxidant activity was studied by lipid peroxidation and DPPH radical scavenging activity assays.The ethyl acetate extract at different concentrations showed a significant percent inhibition of albumin denaturation and percent inhibition of haemolysis when compared with the standard. The extract at 200 and 400 mg/kg b.w. significantly reduced cotton pellet granuloma (P <0.001) and carrageenan paw oedema (P <0.05) when compared with the standard in rats.The extract at different concentrations also showed a significant percent inhibition of lipid peroxidation and DPPH when compared with the standard.Thus, the result indicates that the ethyl acetate extract exhibited significantly good anti-inflammatory and anti-oxidant activities.


Keyword(s)

Anti-inflammatory; Anti-oxidant; Ethnomedicine; Leihruisen; Phytoconstituents.

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