Determination of bioactive phytochemicals, antioxidant and anti-inflammatory activity of Colchicum autumnale L. (Suranjanshireen)

Hailu, Temesgen ; Sharma, Ratnika ; Mann, Sonia ; Gupta, Promila ; Gupta, Rajinder K.; Rani, Archna

Abstract

In Unani System of Medicine Suranjanshireen (Colchicum autumnale L.) is primarily used for the treatment of arthritis, and it belongs to the family of Liliaceae. The current investigation was conducted to evaluate the phytochemical constituents, anti-oxidant, and anti-inflammatory activity of C. autumnale L. Anti-oxidant activity was done using 2, 2'-azinobis (3-ethyl benzothiazoline-6-sulfonic acid), and 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging test. The bioactive compounds of the extracts of C. autumnale were identified by GC-MS and UHPLC-QExactiveOrbitrap. Docking studies were carried out for anti-inflammatory activity.The subjective phytochemicals examination demonstrated the existence of phenols, flavonoids, glycosides, and terpenoids. Whereas the quantitative investigation indicated dichloromethane extract contains the maximum number of phenolic and flavonoids constituents and demonstrated the highest antioxidant activity. GC-MS, and UHPLC- QExactiveOrbitrap investigation of the extracts confirmed the existence of bioactive compounds. Docking analysis revealed that colchicoside (3 demethyl colchicine glucoside) inhibits IL-6 having binding energy -7.1 kcal/mol with an RMSD value of 0.00. Phytochemicals, antioxidant, GC-MS, UHPLC- QExactiveOrbitrap analysis and molecular docking results revealed that the compounds presented in C. autumnale L extracts were accountable for numerous therapeutic uses, for instance, antioxidant, and anti-inflammatory activities.


Keyword(s)

Anti-inflammatory activity; Antioxidant; GC-MS analysis; ICP-MS analysis; UHPLC-QExactiveOrbitrap analysis.

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