Green extraction and in vitro anti-mycobacterial activity of Hydrocotyle sibthorpioides Lam. and Carica papaya L. leaves collected from Assam, India

Das, Sagarika ; Hazarika, Suman ; Sarma, Himangshu ; Goswami, Ashis Kumar; Sharma, Hemanta Kumar


The traditional healers of different parts of India use goat urine for the treatment of tuberculosis. On the basis of ethnomedicinal claims, two plant species namely Hydrocotyle sibthorpioides and Carica papaya were extracted with raw (fresh) and photo-activated goat urine as a menstruum. The present study reports the in vitro antimycobacterial activity of the leaf extracts of H. sibthorpioides and C. papaya against Mycobacterium smegmatis (ATCC 700084 /Mc2155 strain). It was observed that the photo-activated goat urine and raw goat urine leaf extracts could inhibit M. smegmatis. Among all the four extracts, the extract of C. papaya using photo-activated goat urine showed the highest antimycobacterial activity against M. smegmatis.


Ajamutra; Anti-tubercular; Ethnomedicine; Goat urine; Maceration; Northeast India.

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