Volatile constituents of endangered species Nardostachys grandiflora DC. rhizomes from Uttarakhand Himalaya (India)

Purohit, Vijay Kant; Andola, Harish Chandra; Bahuguna, Y M; Gairola, Keshav Chandra; Haider, Syed Zafar; Arunachalam, Kusum

Abstract

The rhizomes of Nardostachys grandiflora DC. syn. N. jatamansi DC. were collected from two alpine Himalayan locations of Uttarakhand (India). The essential oils were obtained by hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) to determine the concentration variation in their constituents. A total of 22 compounds were identified in both the oil samples, accounting 97.6-98.4 %. The major constituents of N. grandiflora oils were characterised as patchoulol (39.1-46.8 %) and calarene (15.1-21.6 %). Due to the higher relative area quantum of patchoulol in N. grandiflora populations growing in Uttarakhand, there is need to develop a propagation protocol for mass multiplication and in-situ and ex-situ conservation of N. grandiflora.


Keyword(s)

Nardostachys grandiflora DC., Valerianaceae, Alpine Himalaya, Essential oil, Patchoulol.

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