In vitro antidiabetic activities of Myanmar medicinal plants: Cassia siamea Lam. and Butea monosperma Roxb.

Thida, Mya ; Moe, The Su; Chan, Khin Nyein; Ei, Shun Lai; Khai, Aye Aye

Abstract

This study aimed to evaluate the antidiabetic potentials of Cassia siamea and Butea monosperma. Cytotoxic activity of test extracts was performed by a hemolytic assay. Estimation of the antidiabetic properties was explored by α-glucosidase and DPP-IV inhibition assays. The glucose transportation activity of test extracts across the yeast cells was expressed by a glucose uptake assay. Non-hemolytic effects of test extracts were shown as lysis per cent less than 15 on RBCs. The inhibition potential of test extracts on α-glucosidase enzyme illustrates that IC50 values (μg/mL) of C. siamea was 76.33±12.2 and B. monosperma was 77.28±2.02. While the IC50 values of the acarbose was 36.76±1.55 μg/mL. In addition, the tested extracts showed the ability to inhibit DPP-IV enzyme activity in a concentration-dependent manner. The IC50 (μg/mL) values of C. siamea, B. monosperma, and the sitagliptin were 117.02±9.73, 103±8.5, and 144.85±13.43, respectively in DPP-IV inhibitory assay. Moreover, the test extracts could transport glucose in yeast cells representing the glucose uptake effectively especially in B. monosperma with a concentration-dependent manner in all tested glucose concentrations. This study provided that the tested extracts promise to possess the antidiabetic potential with non-hemolytic properties, diabetic-enzymes suppressing potency and glucose utilizing ability.


Keyword(s)

Cytotoxic activity; Diabetes mellitus; Dipeptidyl peptidase IV (DPP-IV); Glucose uptake; Insulin

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