Medium Optimization for Xylanase Production by Recombinant Escherichia coli B24

Rashid, W B A B; Malek, R Abd; Wadaan, M A; Elsayed, E A; Sukmawati, D ; El-Enshasy, H A

Abstract

Interest in xylanase enzyme application has led to production of xylanase from recombinant Escherichia coli B24, which is an economic alternative towards higher productivity. Recombinant E. coli used in this study is a ubiquitous bacterium containing xylanase encoding gene from Bacillus halodurans. We investigated xylanase production by recombinant E. coli using classical medium optimization. Six fermentation media had been chosen from literature for xylanase production. Afterwards, the most suitable medium was further optimized by varying the key nutrients. The final optimized cultivation medium consisted of (g.L-1): glucose, 2.5; NH4Cl, 0.4; KH2PO4, 3.0; Na2HPO4, 6.0; MgSO4.7H2O, 1.0. After medium optimization, maximal volumetric xylanase production (600.25 U.mL-1) increased by about 115.22% from the initial un-optimized medium (278.9 U.mL-1).


Keyword(s)

Recombinant; Escherichia coli; Xylanase production; Medium optimization; Fermentation


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