Electro-chemical oxidation and determination of granulocytosis risk inducing drug 4-aminoantipyrine at gold electrode

Abstract

A voltammetric technique for the electrochemical sensing and quantification of granulocytosis risk inducing metabolite of aminopyrine, 4-Aminoantipyrine (4-AAP) at a gold electrode (GE) was developed. The gold electrode showed excellent selectivity and sensitivity towards the oxidation of 4-AAP in phosphate buffer solution (PBS) of pH=3.0. It was observed from the electrochemical studies of scan rate that overall electrode process was diffusion controlled and pH effect suggested equal number of protons and electrons were involved in the electrochemical oxidation of 4-AAP. A linear current response towards the electrochemical oxidation of 4-AAP in the concentration range of 3.8 × 10^-8 M and 1.61 × 10⁻⁷ M was observed with limit of detection of 3.8 × 10^-8 M (S/N = 3). The interference studies showed that the gold electrode exhibited excellent selectivity in the presence of large excess of interferents. The method was employed to recovery study of 4-AAP in spiked urine and serum samples.