Isolation of Pseudomonas aeruginosa for bacterial pigment production and its application on synthetic knitted fabric

Abstract

This study concentrates on the isolation of bio colour producing bacteria from goat droppings and application of its colour pigment in the textile dyeing process. The isolated strain is subjected to biochemical as well as molecular characterization and is identified as Pseudomonas aeruginosa using thin layer chromatography. An optimum condition for production of pigment ‘Pyocyanin (blue-green)’ has been studied by one-factor at a time approach. The pigment (Pyocyanin) is extracted using chloroform, and 0.2N hydrochloric acid. The application of this extracted bio color as coloring agent on polyester fabric has also been studied, and it is found that the color retained on the fabric is yellow, which is different from the extracted pigment colour. Color quality evaluation of dyed polyester fabric has been performed with references to international standard protocols. The color producing is found to be 1-hydroxyphenazine, obtained by the hydrolysis of Pyocyanin at high temperature (130^oC). This 1-hydroxyphenazine imparts the yellow shade to polyester fabric on dyeing.