Biodegradable curative film for medical application

Abstract

An attempt has been made to produce a herbal based silk fibroin – chitosan biodegradable film. Herbs are used for wound healing purpose, silk fibroin for its remarkable bio-medical and mechanical properties and chitosan for its antimicrobial properties. Herbal extracts, such as Aloe barbadensis Mill, (Aloe Vera), Cissus quadrangularis (Pirandai), and Curcuma longa (Turmeric) have been used to impart wound healing effects. The physical and antimicrobial properties have been studied as per the standard methods. The cytotoxic reactivity of the test, and control sample are evaluated under an inverted phase contrast microscope based on the standard. Scanning electron microscope is used to study the morphology of prepared film for process versatility and highly specific surface area. The chemical compound assessments are studied by Fourier Transform Infrared. Microbial growth under the bio films is found to be absent, and test results show better activity against Escherichia coli and Staphylococcus aureus. The blended film shows none cytotoxic reactivity to fibroblast cells and test silk fibroin blended film shows slight cytotoxic reactivity to fibroblast cells after 24 h contact. The achievement of numerical more than 2 is considered as cytotoxic effect. Since the film samples achieve a numerical grade not greater than 2, the samples are considered as non cytotoxic. Microbial growth under the bio films is found to be absent and test results show better activity against Escherichia coli.