In-vitro biocompatibility studies of nanochitosan/ polyvinylpyrrolidone blends on MC3T3-E1 cells showing beneficial effect in bone tissue engineering

Sangeetha, V ; Sudha, P N ; Jayaprabakar, J

Abstract

Chitosan is a naturally occurring polymer with numerous biological properties that can be used in the biomedical field. Chitosan was modified to nanochitosan in this study. The polycationic nature of chitosan interacts with polyanions (TPP) to form nanoparticles. The prepared nanochitosan was mixed with polyvinyl pyrollidone to create binary blends with ratios of 1:1, 1:2, and 2:1. The polymeric blends were interpreted by using the analytical tools FTIR, XRD and SEM. The results of the FTIR and XRD confirm the formation of binary blends with strong electrostatic interaction. The study aims to investigate the compatibility of the prepared blend as tissue regenerative material. The blends were evaluated in-vitro using fluorscent spectroscopy, MTT assay, NRR assay and ALP assay to study its biocompatibility and cell viability. The results revealed that the quick multiplication followed by a moderate increment in cell number of MC3T3-E1 as cells reacted to the prepared material and hence the materials can be used in the regenerative medicine.

Keyword(s)

Cell viability, MTT assay, Nanochitosan, PVP

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