Quantitative estimation and evaluation of anti-inflammatory activity of macromolecules of Boswellia serrata

 

V, Sampath ; Mukhopadhyay, Nabarun ; Pai, Sameer ; N, Dilip ; M, Sai Sandeep ; Babu, U V ; Lobo, Richard ; Pai, Vasudev

Abstract

The present study was aimed to isolate and perform qualitative analysis of macromolecules present in Boswellia serrata followed by evaluation of their anti-inflammatory activity by in-vitro IL-6 ELISA study. The oleo gum resin of B. serrata was pretreated with hexane, methanol and the obtained residue was extracted with water and the final dried powder was taken for macromolecule analysis. The macromolecules which comprise of polysaccharides, mucilages and proteins were quantitatively estimated and evaluated for their anti-inflammatory activity by in-vitro IL-6 ELISA study. From the current study, it was found that the macromolecules present in B. serrata majorly comprise of polysaccharides and mucilages with moderate quantity of proteins. The quantitative analysis of polysaccharides, mucilages and proteins were found 35.91%, 34% and 14.29%. From the in-vitro IL-6 ELISA study, it was found that the macromolecules showed 82.63% inhibition of IL-6. The identified macromolecules from B. serrata showed significant anti-inflammatory activity by inhibition of IL- 6 and further need to confirm by in vivo study to qualify B. serrata macromolecules as a promising anti-inflammatory agent.

Keyword(s)

Anti-inflammatory activity; Boswellia serrata; IL-6 ELISA; Macromolecules; MTT assay

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