Investigation of Cytotoxic Effect of Black Mulberry (Morus nigra L.) Fruit

Dalkilic, Semih ; Dalkilic, Lütfiye Kadioglu; İnci, Sule ; Korkmaz, İsmail ; Kirbag, Sevda


The aim of this study was to determine the cytotoxic effect of black mulberry fruit extract (Morus nigra L.) on human breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. The extract prepared from mulberry (M. nigra) fresh fruit was applied to cancer cell lines in 5 different concentrations (1/10, 1/25, 1/50, 1/75 and 1/100) for 72 hours. At the end of the incubation period, cell viability was determined by MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay. Results were compared with negative and positive control groups. The effect of mulberry (M. nigra) fruit extract on cell viability was determined depending on the concentration applied; extract showed a significant cytotoxic effect on PC3 cell line at the 1/10 concentration compared to the control group, but not detected any effect at 1/25, 1/50, 1/75 and 1/100 concentrations. In the MDA-MB-231 cell line, there was a high cytotoxic effect at a concentration of 1/10 compared to the control group, while a lower effect was observed at 1/25, 1/50, 1/75 and 1/100 concentrations. The data obtained from the study showed that extract prepared from black mulberry fruit has cytotoxic effect especially against breast cancer cells.


Breast cancer; Cytotoxicity; Morus nigra L.; MTT assay; Prostate cancer

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