Pongamol from the seeds of Tephrosia purpurea exert anti-cancer activity against cervical cancer cell line

Abstract

Tephrosia purpurea belongs to the family Fabaceae, is used for the treatment of inflammation, diabetes, cancer, elephantitis, haemmaroids, anaemia, dysmenorrhea, chronic fever, boils, gingivitis etc. In the present study, three compounds (TP-1, TP-3 and TP-5) were isolated from EETP. Quantity of TP-3 (6.05 %) in EETP was determined by HPLC. In-vitro anti-cancer activity of EETP and isolated compound TP-3 on SiHa cells (cervical cancer cell lines) as well as PBMCs (peripheral blood mononuclear cells) were evaluated by MTT (3-(4,5- di methyl thiazol -2-yl)-2,5-di phenyl tetrazolium bromide) assay. Spectroscopic analysis and physical properties, structure of isolated compounds TP-1, TP-3 and TP-5 were characterized as teclenone, pongamol and β-sitosterol respectively.

Isolated compound TP-3 showed cytotoxicity against SiHa cells with the IC₅₀ 33.06 µgmL^-1. TP-3 showed cytotoxicity at all the concentrations but significant activity was observed at the concentration range from 20-50 µgmL^-1 as compared to control. Maximum cytotoxicity (72.75%) was observed at the concentration of 50 µgmL^-1. TP-3 showed non-significant cytotoxicity against PBMCs cells at all the concentrations except at 50 µgmL^-1 (cytotoxicity 24.19%). EETP showed significant cytotoxic activity (IC₅₀ value 113.63 µgmL^-1) against SiHa cells at the concentration range from 75-150 µgmL^-1 as compared to control. Maximum cytotoxicity (75.34%) was observed at the concentration of 150 µgmL^-1.