In vitro study of antibacterial activity of Kanocha seeds (Phyllanthus maderaspatensis) against some gram-positive and gram-negative bacterial strains

Abstract

Kanocha is the dried seeds of Phyllanthus maderaspatensis of family Phyllanthaceae. It has been long utilising in Unani system of medicine to cure many ailments including genitourinary infectious diseases. In the current investigation, the aqueous, alcoholic and hydroalcoholic extracts of Kanocha seeds were screened for their antibacterial actions against both gram-positive (Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Corynebacterium xerosis and Bacillus cereus) and gram-negative (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris) bacterial strains compared with the standard Drug: Ciprofloxacin (SD060) 5 μg/disk for gram-positive bacterial strains and Gentamicin (SD016) 10 μg/disk for gram-negative bacterial strains using Zone of Inhibition (ZOI) with the help of Agar well method and minimum inhibitory concentration (MIC) & minimum bactericidal concentration (MBC) with the help of Nutrient Broth method. The data was analysed using Gpad INSTAT programming, one-way ANOVA and post-test Bonferroni. Alcoholic and hydroalcoholic extract showed significant antibacterial activity than the aqueous extract but not up to the mark as compared to standard group indicating that the alcoholic and hydroalcoholic extract has the capability of extracting more phytochemicals than aqueous extract which are responsible for
their antibacterial activity. It could be concluded that the present drug possesses antibacterial property.