Impact of GA3 encapsulated nanosilica on maize seed viability

Chourasiya, Vinay Kumar; Shukla, Prabha Shankar; Maurya, Chhote Lal; Prasad, Birandra ; Choudhary, Rakesh ; Pandey, Deepankar ; Kushwaha, Paras

Abstract

The nanoparticles have the potential ability of the passing cell membrane because of their nano sizes. The SiO2 nanoparticles are one of the major and frequently used engineered oxide nanoparticles. In present investigation, the potential effect of SiO2 (10-20 nm) nanoparticles on maize seed viability were studied. We observed quick result of seed viability and also increase the seed viability percentage in presence of GA3 loaded silica nanoparticles. Three concentration of (0.5, 0.2 and 0.1%) tetrazolium salts were also used for staining of living tissue. In the present experiment seeds conditioned in 150 pp GA3 encapsulated nanosilica and tested in 0.5% TZ salt gave maximum viable seeds which was due to the increased availability of GA3 and showed quick staining over 0.2% and 0.1% concentration. Among the varieties, var. Navin imbibed in 150 ppm GA3 loaded nanosilica showed higher value of viable seed under 0.5% tetrazolium solution by better availability of GA3. Gibberellic acid enhances metabolic activity of seed by secretion of α-amylase enzyme which is important for quick staining of embryonic tissue.

Keyword(s)

GA3-Gibberellic Acid, Nanosilica, Seed, Tetrazolium, Viability


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