E. coli AB 1157 susceptibility test, MTT assay on MCF-7 and HeLa cell lines of root and leaf fractions of Viburnum Linn. species

Ponnudurai, K ; Prabhu, K ; Rani, S Shobha; Murthy, M Srinivasa

Abstract

The aim of this study was to evaluate mutagenic bacterial susceptibility and cytotoxic potency of Viburnum coriaceum
Blume and Viburnum erubescens Wall.ex DC in order to report the actual chemotherapeutic potentials of these two species.
The methanolic (80%) leaf and chloroform root extracts of Viburnum Linn. Species were tested for their bacterial strain
based cytotoxicity employing Agar diffusion method suing E. coli AB 1157 strain. Also, the MTT assay was carried out
employing MCF-7 breast cancer cell lines and HeLa cervical cell lines. It was started with IC50 value determination of the
selected test extracts from the results of bacterial strain based cytotoxicity. Upon increase in concentration up to 1000
μg/mL in agar diffusion cytotoxicity studies VCMLE, VEMLE and VCCRE had shown diameter of inhibition zone 10 mm,
9 mm and 10 mm respectively. Among other extracts, the VEMLE and VCCRE were selected to go ahead with anticancer
activity by MTT assay. The potentials of extracts through cytotoxicity mechanism had produced 30-40% protection against
cancer cell lines. It was concluded that VEMLE and VCCRE had produced the cytotoxic effect on E. coli AB 1157 strain.
and were selected for the cytotoxic studies. The effects exhibited by the selected extracts may be due to the presence
of diverse number of active constituents present in Viburnum Linn species also may be to the presence of
unreported constituents.


Keyword(s)

Cytotoxicity; HeLa; MTT assay; MCF-7; Susceptibility test; Viburnum

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